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The strain YL 3g-3, which was capable of producing e:tracaulu cytosine dermiaase, was bolated and taxonoakal y ezanbKd. The bobaed steam was identified to be Badfbrs po(ymyxa YL 31-3. The optimal eoadi oas for the enzyme production from BoeUus polymyxa YL 31-3 were Investigated. The enzyme production was raehed nraxinm level la the media contaii?g 0.5% glucose, 0.2% beef extract, 0.5% Naa and 0.1% KH3PO4 (pH 6.0). And the enzym showed the highest activity when the strsis YL 38-3 was cultivated at 35°C for 24 bows uadcc the Initial pH 6.0. By the additions of peptone the extracelahr enzyme production was inhibited, nseanwhk the inlraeeBnlar enzyme production was high stimulated. It was, ther fore, deduced that peptone was related to the secretion oeckaaism of the enzyme from this bacterial cell.
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